Kitabı oku: «Studies in the Theory of Descent, Volume I», sayfa 8

APPENDIX I. EXPERIMENTS

Experiments with Araschnia Levana

1. Bred from eggs laid by a female of the winter form on 12th-15th May, 1868, in a breeding-cage. The caterpillars emerged on 20th-22nd May, and pupated on 7th-9th June. The pupæ, kept at the ordinary temperature, produced: —

All these butterflies were of the Prorsa type, 3 females having a considerable amount of yellow, but none with so much as figs. 3, 4, 7, 8, or 9. Pl. I.

2. August 12th, 1868, found larvæ of the third generation, which pupated at the beginning of September, and were kept in a room not warmed. In September three butterflies emerged in the Prorsa form, the remainder hibernating and producing, after being placed in a heated room at the end of February, from the 1st to the 17th of March, 1869, more butterflies, all of the Levana form.

3. Larvæ found on the 17th June, 1869, were sorted according to colour; the yellow ones, with light brown spines, produced, at the ordinary temperature, on 8th-12th July, 13 butterflies, 12 of which showed the ordinary Prorsa type, and one, a male, possessing more yellow than fig. 3, Pl. I., must be considered as a Porima type.

4. From caterpillars of the second generation, found at the same time as those of Exp. 3, 30 pupæ were placed in the refrigerator (temperature 8°-10° R.) on June 25th. When the box was opened on August 3rd, almost all had emerged, many being dead, and all, without exception, were of the intermediate form (Porima), although nearer the Prorsa than the Levana type.

5. A large number of caterpillars of the second generation, found at the same time, pupated, and were kept at a high summer temperature. After a pupal period of about 19 days, some 70 butterflies emerged from 28th June to 5th July, all of the Prorsa form, with the exception of 5, which were strongly marked with yellow (Porima).

6. The 70 butterflies of the foregoing experiment were placed in an enclosure 6 feet high, and 8 feet long, in which, during warm weather, they freely swarmed on flowers. Copulation was only once observed, and but one female laid eggs on nettle on July 4th. At the high summer temperature prevailing at the time, these eggs produced butterflies after 30–31 days (third generation). All were Prorsa, with more or less yellow; among 18 none were completely Porima.

7. Young larvæ of the fourth generation, found on the 8th of August, were reared in a hothouse (17°-20° R.). They pupated on 21st-23rd August. Of these: —

A. 56 pupæ were placed on ice (0°-1° R.) for five weeks, and then allowed to hibernate in a room not warmed. In April, 1870, they all gave the Levana form, with the exception of a single Porima.

B. About an equal number of pupæ were placed in the hothouse, but without any result; for, notwithstanding a temperature of 12°-24° R., not a single butterfly emerged in the course of October and November. The pupæ were then allowed to hibernate in an unheated room, and in April and May gave nothing but Levana.

8. Caterpillars of the second generation, found at the beginning of June, 1870, pupated on 13th-15th June, and gave, at the ordinary temperature, on June 29th-30th, 7 butterflies of the Prorsa form.

9. Pupæ of the same (second) generation were placed immediately after pupation on June 18th, 1870, in a refrigerator (0°-1° R.), and after remaining there four weeks (till July 18th) gave, at the ordinary summer temperature: —

Of these 20 butterflies only 5 were of the pure Prorsa form.

10. Full grown larvæ of the fourth generation, found on August 20th, 1870, pupated on August 26th to September 5th. The pupæ were divided into three portions: —

A. Placed in the hothouse (12°-25° R.), immediately after pupation and left there till October 20th. Of about 40 pupæ only 4 emerged, 3 of which were Prorsa and 1 Porima. The remaining pupæ hibernated and all changed into Levana the following spring.

B. Kept in a room heated to 6°-15° R. from November. Not a single specimen emerged the same year. This lot of pupæ were added to C from November.

C. Placed on ice for a month immediately after pupation; then, from September 28th to October 19th in the hothouse, where no more butterflies emerged. The pupæ hibernated, together with those from lot B, in a room heated by water to 6°-15° R., and gave: —

The exact record of the time of emergence is interesting, because it is thereby rendered apparent that different individuals respond more in different degrees to a higher than to the ordinary temperature. Whilst with many an acceleration of development of 1–2 months occurred, others emerged in April and May, i.e. at the time of their appearance in the natural state.

11. Reared the second generation from eggs of the first generation. Emerged from the eggs on June 6th, 1872, pupated on July 9th. The pupæ were placed on ice (0°-1° R.) from July 11th till September 11th, and then transferred to a hothouse, where all emerged: —

It must be pointed out, however, that among those specimens marked as “Levana” there were none which entirely corresponded with the natural Levana, or which indeed approximated so nearly to this form as did some of the specimens in Exp. 9. All were larger than the natural Levana, and possessed, notwithstanding the large amount of yellow, more black than any true Levana. In all artificially bred Levana the black band of the basal half of the hind wings is always interrupted with yellow, which is seldom the case with true Levana. The whole appearance of the artificial Levana is also coarser, and the contour of the wings somewhat different, the fore-wings being broader and less pointed. (See figs. 7 to 9, Pl. I.).

12. Larvæ of the fourth generation, found on September 22nd, 1872, were divided into two portions: —

A. Placed for pupation in an orchid-house at 12°-25° R., and allowed to remain there till December. In spite of the high temperature not a single butterfly emerged during this time, whilst pupæ of Vanessa C-album and Pyrameis Atalanta, found at the same time, and placed in the same hothouse, emerged in the middle of October. From the middle of December the pupæ were kept in an unheated room, and they emerged very late in the spring of 1873, all as Levana: —

B. Kept in an unheated room during the winter. The butterflies emerged from the 28th of May, all as Levana.

Experiments with Pierinæ

13. Females of Pieris Rapæ, captured in April, laid eggs on Sisymbrium Alliaria. From these caterpillars were obtained, which pupated on 1st-3rd June. The pupæ were placed on ice from June 3rd till September 11th (0°-1° R.), and from September 11th till October 3rd in the hothouse (12°-24° R.), where there emerged: —

All these were sharply impressed with the characters of the winter form, the females all strongly yellow on the upper side, the males pure white; on the under side a strong black dusting on the hind wings, particularly on the discoidal cell. One pupa did not emerge in the hothouse, but hibernated, and gave in a heated room on January 20th, 1873, a female, also of the winter form.

14. Females of Pieris Napi, captured on 27th-28th April, 1872, laid eggs on Sisymbrium Alliaria. The larvæ bred from these pupated on May 28th to June 7th. The pupæ, shortly after transformation, were placed on ice, where they remained till Sept. 11th (three months). Transferred to the hothouse on October 3rd, they produced, up to October 20th, 60 butterflies, all with the sharply-defined characters of the winter form. The remaining pupæ hibernated in a room, and produced: —

15. Several butterflies from Exp. 14, which emerged in May, 1873, were placed in a capacious breeding-house, where they copulated and laid eggs on rape. The caterpillars fed on the living plants in the breeding-house, and after pupation were divided into two portions: —

A. Several pupæ, kept at the ordinary summer temperature, gave butterflies on July 2nd, having the characters of the summer form.

B. The remainder of the pupæ were placed on ice immediately after transformation, and remained over three months in the refrigerator (from July 1st till October 10th). Unfortunately most of them perished through the penetration of moisture into the box. Only 8 survived, 3 of which emerged on the 20th of October as the winter form; the others hibernated in an unheated room, and emerged at the beginning of June, 1874. All 5 were females, and all exhibited the characters of the winter form. Notwithstanding a pupal period of eleven months, they did not possess these characters to a greater extent than usual, and did not, therefore, approximate to the parent-form Bryoniæ.

16. On June 12th, 1871, specimens of Pieris Napi, var. Bryoniæ, were captured on a mountain in the neighbourhood of Oberstorf (Allgäuer Alpen), and placed in a breeding-house, where they flew freely about the flowers; but although copulation did not take place, several females laid eggs on the ordinary garden cabbage. From these caterpillars were hatched, which at all stages of growth were exactly like those of the ordinary form of Napi. They throve well until shortly before pupation, when a fungoid epidemic decimated them, so that from 300 caterpillars only about 40 living pupæ were obtained. These also completely resembled the ordinary form of Napi, and showed the same polymorphism, some being beautifully green, others (the majority) straw yellow, and others yellowish grey. Only one butterfly emerged the same summer, a male, which, by the black dusting of the veins on the margin of the wings (upper side), could be with certainty recognized as var. Bryoniæ. The remaining pupæ hibernated in a heated room, and gave, from the end of January to the beginning of June, 10 males and 5 females, all with the characters of the var. Bryoniæ. They emerged: —

We here perceive that the tendency to accelerate development through the action of warmth is, in this case, also very different in different individuals. Of the 16 butterflies only 1 kept to the normal period of development from July 27th to June 3rd, fully ten months; all the others had this period abbreviated, 1 male to eleven days, 8 specimens to six months, 4 to seven months, 2 to eight months, and 1 to nine months.

APPENDIX II

Experiments with Papilio Ajax. ⁵⁸

From eggs of var. Telamonides laid on the last of May larvæ were obtained, which gave on June 22nd-26th, 122 pupæ. These, as fast as formed, were placed on ice in the refrigerator in small tin boxes, and when all the larvæ had become transformed the pupæ were transferred to a cylindrical tin box (4 in. diam. and 6 in. high), and packed in layers between fine shavings. The tin box was set in a small wooden one, which was put directly on the ice and kept there till July 20th. From that date, by an unfortunate accident, the box, instead of being kept on the surface of the ice in an ice-house, as was intended, was placed on straw near the ice, so that the action of the cold was modified, the outside pupæ certainly experiencing its full effects, but the inside ones were probably at a somewhat higher temperature. The ice failed on August 20th, so that the pupæ had been subjected to an equable low temperature in the refrigerator for three to four weeks, and to a lesser degree of cold in the ice-house for five weeks, the temperature of the last place rising daily, as the ice had all thawed by August 20th. On opening the box it was found (probably owing to the cold not having been sufficiently severe) that the butterflies had commenced to emerge. Twenty-seven dead and crippled specimens were removed, together with several dead pupæ. One butterfly that had just emerged was taken out and placed in a box, and when its wings had fully expanded it was found to be a “Telamonides of the most pronounced type.” The experimenter then states: – “Early in the morning I made search for the dead and rejected butterflies, and recovered a few. It was not possible to examine them very closely from the wet and decayed condition they were in, but I was able to discover the broad crimson band which lies above the inner angle of the hind wings, and which is usually lined on its anterior side with white, and is characteristic of either Walshii or Telamonides, but is not found in Marcellus. And the tip only of the tail being white in Walshii, while both tip and sides are white in Telamonides, enabled me to identify the form as between these two. There certainly were no Walshii, but there seemed to be a single Marcellus, and excepting that all were Telamonides.”

The remaining pupæ were kept in a light room where 3 Telamonides emerged the following day, and by September 4th 14 specimens of the same variety had emerged, but no Marcellus or intermediate forms. From the 4th to the 20th of September a few more Telamonides appeared, but between the 4th and 15th of the month 12 out of 26 butterflies that had emerged were intermediate between Telamonides and Marcellus, some approximating to one form and some to the other form. The first pure Marcellus appeared on September 4th, and was followed by one specimen on the 6th, 8th, 13th and 15th respectively. From this last date to October 3rd, 6 out of 10 were Marcellus and 3 intermediate. On September 3rd, a specimen intermediate between Telamonides and Walshii emerged, “in which the tails were white tipped as in Walshii, but in size and other characters it was Telamonides, though the crimson band might have belonged to either form.” Butterflies continued to emerge daily up to September 20th, after which date single specimens appeared at intervals of from four to six days, the last emergence being on October 16th. Thus, from the time the box was removed from the ice-house, the total period of emerging was fifty-seven days, some specimens having emerged before the removal of the box. With specimens of P. Ajax which appear on the wing the first season the natural pupal period is about fourteen days, individuals rarely emerging after a period of four to six weeks.

Between August 20th and October 16th, the 50 following butterflies emerged: —

All these butterflies were very uniform in size, being about that of the ordinary Telamonides. The specimens of Telamonides especially were “strongly marked, the crimson band in a large proportion of them being as conspicuous as is usual in Walshii, and the blue lunules near the tail were remarkably large and bright coloured. Of the Marcellus, in addition to the somewhat reduced size, the tails were almost invariably shorter than usual and narrower, and instead of the characteristic single crimson spot, nearly all had two spots, often large. In all these particulars they approach Telamonides.”

Adding to the Telamonides which emerged after August 20th most of those specimens which were found dead in the box at that date, the total number of this form is thus brought up to nearly 50. Of the 122 pupæ with which Mr. Edwards started, 28 remained in a state fit for hibernation, several having died without emerging. Previous experiments had shown that 28 out of 122 pupæ is not an unreasonable number to hibernate, so that the author concludes that the butterflies which emerged the same season would have done so naturally, and the effect of the artificial cold was not “to precipitate the emerging of any which would have slept” till the following spring. Now under ordinary circumstances all the butterflies which emerged the same season would have been of the Marcellus form, so that the cold changed a large part of these into the form Telamonides, some (probably from those pupæ which experienced the lowest temperature) being completely changed, and others (from those pupæ which were only imperfectly subjected to the cold) being intermediate, i. e., only partly changed. It appears also that several pupæ experienced sufficient cold to retard their emergence and stunt their growth, but not enough to change their form, these being the 13 recorded specimens of Marcellus. Had the degree of cold been equal and constant, the reversion would probably have been more complete. The application of cold produced great confusion in the duration of the pupal period, the emergence, instead of taking place fourteen days after the withdrawal of the cold, as might have been expected from Dr. Weismann’s corresponding experiment with Pieris Napi (Appendix I. Exps. 13 and 14), having been extended over more than two months.

From the results of this experiment it must be concluded that Telamonides is the primary form of the species.

Additional Experiments with Papilio Ajax

[Communicated by Mr. W. H. Edwards, November 18th, 1879.]

Exp. 1. – In 1877 chrysalides of P. Ajax and Grapta Interrogationis (the eggs laid by females of the form Fabricii) were experimented upon; but the results were not satisfactory, for the reason that the author having been absent from home most of the time while the pupæ were in the ice-box, on his return found the temperature above 5°-6° R. And so far as could be told, the ice had been put in irregularly, and there might have been intervals during which no ice at all was in the box. Six chrysalides of the Grapta so exposed produced unchanged Umbrosa, the co-form with Fabricii. But all chrysalides from the same lot of eggs, and not exposed to cold, also produced Umbrosa. Nothing was learnt, therefore, respecting this species.

But chrysalides of Ajax, exposed at same time, did give changed butterflies to some extent. From a lot of 8, placed in the box when under twelve hours from pupation, and left for twenty-four days, there came 5 males and 3 females. Of these was 1 Telamonides in markings and coloration, and all the rest were between Marcellus and Telamonides. Two other chrysalides on ice for twenty-three days gave Telamonides, but 3 more exposed twenty-six days, and all one hour old when put on ice, were unchanged, producing Marcellus.

During the same season 6 other Ajax chrysalides were placed in the box, and kept at about 0°-1° R. One was one hour old, and remained for five days; 1 was one hour old, and remained for two days and three-quarters; 3 at three hours old for eight days; and 1 (age omitted), six days. All these gave unchanged butterflies of the form Marcellus.

Exp. 2. – In May, 1878, many chrysalides were placed in the ice-box, being from eggs laid by Ajax, var. Walshii. The youngest were but ten to fifteen minutes from pupation, and were soft; others at intervals up to twenty-four hours (the chrysalis is hard at about twelve hours); after that, each day up to eight days after pupation. All were removed from the box on the same day, 28th May. The exposure was from nineteen to five days, those chrysalides which were put on ice latest having the shortest exposure. The author wished to determine if possible whether, in order to effect any change, it was necessary that cold should be applied immediately after pupation or if one or several days might intervene between pupation and refrigeration. Inasmuch as no colour begins to show itself in the pupæ till a few hours, or at most a day or two, before the butterfly emerges, it was thought possible that cold applied shortly before that time would be quite as effective as if applied earlier and especially very soon after pupation. The result was, that more than half of the chrysalides exposed before they had hardened died: 1 exposed at ten minutes, 2 at one hour, 1 at two hours, 2 at three hours after pupation. On the other hand 1 at fifteen minutes produced a butterfly, 1 at two hours, another at twelve hours. The temperature was from 0°-1° R. most of the time, but varied somewhat each day as the ice melted. The normal chrysalis period is from eleven to fourteen days, in case the butterfly emerges the same season, but very rarely an individual will emerge several weeks after pupation.

On the 14th day after taking the pupæ from the ice, 1 Telamonides emerged from a chrysalis which had been placed in the ice-box three days after pupation, and was on ice sixteen days.

On 19th day, 1 Telamonides emerged from a pupa put on the ice twelve hours after pupation, and kept there eleven days.

On 19th day, 1 Walshii emerged from a pupa two hours old, and on ice eleven days.

All the rest emerged Marcellus, unchanged, but at periods prolonged in a surprising way.

Five chrysalides lived through the winter, and all gave Telamonides in the spring of 1879.

It appeared, therefore, that the only effect produced by cold in all chrysalides exposed more than three days after pupation was to retard the emergence of the butterfly. But even in some of these earliest exposed, and kept on the ice for full nineteen days, the only effect seemed to be to retard the butterfly.

Exp. 3. – In June, 1879, eggs of the form Marcellus were obtained, and in due time gave 104 chrysalides. Of these one-third were placed in the ice-box at from twelve to twenty-four hours after pupation, and were divided into 3 lots.

Temperature 0°-1° R. most of the time, but varying somewhat as the ice melted. (Both in 1878 and 1879 Mr. Edwards watched the box himself, and endeavoured to keep a low temperature.)

Of the 69 chrysalides not exposed to cold, 34 gave butterflies at from eleven to fourteen days after pupation, and 1 additional male emerged 11th August, or twenty-two days at least past the regular period of the species.

Of the iced chrysalides, from lot No. 1 emerged 4 females at eight days and a half to nine days and a half after removal from the ice, and 5 are now alive (Nov. 18) and will go over the winter.

From lot No. 2 emerged 1 male and 5 females at eight to nine days; another male came out at forty days; and 5 will hibernate.

From lot No. 3 emerged 4 females at nine to twelve days; another male came out at fifty-four days; and 6 were found to be dead.

In this experiment the author wished to see as exactly as possible – First, in what points changes would occur. Second, if there would be any change in the shape of the wings, as well as in markings or coloration – that is, whether the shape might remain as that of Marcellus, while the markings might be of Telamonides or Walshii; a summer form with winter markings. Third, to ascertain more closely than had yet been done what length of exposure was required to bring about a decided change, and what would be the effect of prolonging this period. After the experiments with Phyciodes Tharos, which had resulted in a suffusion of colour, the author hoped that some similar cases might be seen in Ajax. The decided changes in 1878 had been produced by eleven and sixteen days’ cold. In 1877, an exposure of two days and three-quarters to eight days had failed to produce an effect.

From these chrysalides 11 perfect butterflies were obtained, 1 male and 10 females. Some emerged crippled, and these were rejected, as it was not possible to make out the markings satisfactorily.

From lot No. 1, fourteen days, came: —

1 female between Marcellus and Telamonides.

2 females, Marcellus.

These 2 Marcellus were pale coloured, the light parts a dirty white; the submarginal lunules on hind wings were only two in number and small; at the anal angle was one large and one small red spot; the frontal hairs were very short. The first, or intermediate female, was also pale black, but the light parts were more green and less sordid; there were 3 large lunules; the anal red spot was double and connected, as in Telamonides; the frontal hairs short, as in Marcellus. These are the most salient points for comparing the several forms of Ajax. In nature, there is much difference in shape between Marcellus and Telamonides, still more between Marcellus and Walshii; and the latter may be distinguished from the other winter forms by the white tips of the tails. It is also smaller, and the anal spot is larger, with a broad white edging.

From lot No. 2, twenty days, came: —

1 female Marcellus, with single red spot.

1 female between Marcellus and Telamonides; general coloration pale; the lunules all obsolescent; 2 large red anal spots not connected; frontal hairs medium length, as in Telamonides.

1 female between Marcellus and Telamonides; colour bright and clear; 3 lunules; 2 large red spots; frontal hairs short.

1 female Telamonides; colours black and green; 4 lunules; a large double and connected red spot; frontal hairs medium.

2 female Telamonides; colours like last; 3 and 4 lunules; 2 large red spots; frontal hairs medium.

From lot No. 3, twenty-five days, came: —

1 male Telamonides; clear colours; 4 large lunules; 1 large, 1 small red spot; frontal hairs long.

1 female Telamonides; medium colours; 4 lunules; large double connected red spot; frontal hairs long.

In general shape all were Marcellus, the wings produced, the tails long.

From this it appeared that those exposed twenty-five days were fully changed; of those exposed twenty days, 3 were fully, 2 partly, 1 not at all; and of those exposed fourteen days, 1 partly, 2 not at all.

The butterflies from this lot of 104 chrysalides, but which had not been iced, were put in papers. Taking 6 males and 6 females from the papers just as they came to hand, Mr. Edwards set them, and compared them with the iced examples.

Of the 6 males, 4 had 1 red anal spot only, 2 had 1 large 1 small; 4 had 2 green lunules on the hind wings, 2 had 3, and in these last there was a 4th obsolescent, at outer angle; all had short frontal hairs.

Of the 6 females, 5 had but 1 red spot, 1 had 1 large 1 small spot; 5 had 2 lunules only, 1 had 3; all had short frontal hairs.

Comparing 6 of the females from the iced chrysalides, being those in which a change had more or less occurred, with the 6 females not iced:

1. All the former had the colours more intense, the black deeper, the light, green.

2. In 5 of the former the green lunules on hind wings were decidedly larger; 3 of the 6 had 4 distinct lunules, 1 had 3, 1 had 3, and a 4th obsolescent. Of the 6 females not iced none had 4, 2 had 2, and a 3rd, the lowest of the row, obsolescent; 3 had 3, the lowest being very small; one had 3, and a 4th, at outer angle, obsolescent.

3. In all the former the subapical spot on fore wing and the stripe on same wing which crosses the cell inside the common black band, were distinct and green; in all the latter these marks were either obscure or obsolescent.

4. In 4 of the former there was a large double connected red spot, and in one of the 4 it was edged with white on its upper side; 2 had 1 large and 1 small red spot. Of the latter 5 had 1 spot only, and the 6th had 1 spot and a red dot.

5. The former had all the black portions of the wing of deeper colour but less diffused, the bands being narrower; on the other hand, the green bands were wider as well as deeper coloured. Measuring the width of the outermost common green band along the middle of the upper medium interspace on fore wing in tenths of a millimetre, it was found to be as follows:

Measuring the common black discal band across the middle of the lower medium interspace on fore wing:

In other words the natural examples were more melanic than the others.

No difference was found in the length of the tails or in the length and breadth of wings. In other words, the cold had not altered the shape of the wings.

Comparing 1 male iced with 6 males not iced:

1. The former had a large double connected red anal spot, edged with white scales at top. Of the 6 not iced, 3 had but 1 red spot, 2 had 1 large 1 small, 1 had 1 large and a red dot.

2. The former had 4 green lunules; of the latter 3 had 3, 3 had only 2.

3. The former had the subapical spot and stripe in the cells clear green; of the latter 1 had the same, 5 had these obscure or obsolescent.

4. The colours of the iced male were bright; of the others, 2 were the same, 4 had the black pale, the light sordid white or greenish-white.

Looking over all, male and female, of both lots, the large size of the green submarginal lunules on the fore wings in the iced examples was found to be conspicuous as compared with all those not iced, though this feature is included in the general widening of the green bands spoken of.

In all the experiments with Ajax, if any change at all has been produced by cold, it is seen in the enlarging or doubling of the red anal spot, and in the increased number of clear green lunules on the hind wings. Almost always the frontal hairs are lengthened and the colour of the wings deepened, and the extent of the black area is also diminished. All these changes are in the direction of Telamonides, or the winter form.

That the effect of cold is not simply to precipitate the appearance of the winter form, causing the butterfly to emerge from the chrysalis in the summer in which it began its larval existence instead of the succeeding year, is evident from the fact that the butterflies come forth with the shape of Marcellus, although the markings may be of Telamonides or Walshii. And almost always some of the chrysalides, after having been iced, go over the winter, and then produce Telamonides, as do the hibernating pupæ in their natural state. The cold appears to have no effect on these individual chrysalides.⁵⁹

With every experiment, however similar the conditions seem to be, and are intended to be, there is a difference in results; and further experiments – perhaps many – will be required before the cause of this is understood. For example, in 1878, the first butterfly emerged on the fourteenth day after removal from ice, the period being exactly what it is (at its longest) in the species in nature. Others emerged at 19–96 days. In 1879, the emergence began on the ninth day, and by the twelfth day all had come out, except three belated individuals, which came out at twenty, forty, and fifty-four days. In the last experiment, either the cold had not fully suspended the changes which the insect undergoes in the chrysalis, or its action was to hasten them after the chrysalides were taken from the ice. In the first experiment, apparently the changes were absolutely suspended as long as the cold remained.

It might be expected that the application of heat to the hibernating chrysalides would precipitate the appearance of the summer form, or change the markings of the butterfly into the summer form, even if the shape of the wings was not altered; that is, to produce individuals having the winter shape but the summer markings. But this was not found to occur. Mr. Edwards has been in the habit for several years of placing the chrysalides in a warm room, or in the greenhouse, early in the winter, thus causing the butterflies to emerge in February, instead of in March and April, as otherwise they would do. The heat in the house is 19° R. by day, and not less than 3.5° R. by night. But the winter form of the butterfly invariably emerged, usually Telamonides, occasionally Walshii.